A COMPARATIVE STUDY ON THE BIOSURFACTANT PRODUCING BACTERIA FROM OIL CONTAMINATED WATER

Abstract

Bio surfactants are a structurally diverse group of surface- active molecules synthesized
by microorganisms. It’s have attracted much attention because of advantageous
characteristics such as structural diversity, low toxicity, higher biodegradability, better
environmental compatibility and the higher substrate selectivity. Oil spreading
technique, blood hemolysis test, foaming activity, and emulsification activity were
performed for the screening of the biosurfactant producing bacteria, and found positive
oil spreading technique in bacterial strain Bacillus subtilis and Pseudomonas aeruginosa.
Pseudomonas aeruginosa has high emulsifying activity and it was 29.62 % where as in
Bacillus subtilis 20.68 %. Bacillus subtilis and Pseudomonas aeruginosa showed blood
hemolysis test and it was observed that foaming stability in Pseudomonas aeruginosa is
higher than Bacillus subtilis. Pseudomonas aeruginosa showed gram negative and Bacillus
subtilis showed gram negative and they are in rod and circular shape respectively
Bacillus subtilis and Pseudomonas aeruginosa both the organism have higher growth rate
at pH 7 and Pseudomonas aeruginosa shows more growth than Bacillus. Pseudomonas
aeruginosa shows higher growth rate as compare to Bacillus subtilis at 370C and the
Pseudomonas aeruginosa shows better growth rate than Bacillus subtilis at 37°C