A COMPARATIVE STUDY ON THE BIOSURFACTANT PRODUCING BACTERIA FROM OIL CONTAMINATED WATER

Abstract

Bio surfactants are a structurally diverse group of surface- active molecules synthesized by microorganisms. It’s have attracted much attention because of advantageous characteristics such as structural diversity, low toxicity, higher biodegradability, better environmental compatibility and the higher substrate selectivity. Oil spreading technique, blood hemolysis test, foaming activity, and emulsification activity were performed for the screening of the biosurfactant producing bacteria, and found positive oil spreading technique in bacterial strain Bacillus subtilis and Pseudomonas aeruginosa. Pseudomonas aeruginosa has high emulsifying activity and it was 29.62 % where as in Bacillus subtilis 20.68 %. Bacillus subtilis and Pseudomonas aeruginosa showed blood hemolysis test and it was observed that foaming stability in Pseudomonas aeruginosa is higher than Bacillus subtilis. Pseudomonas aeruginosa showed gram negative and Bacillus subtilis showed gram negative and they are in rod and circular shape respectively Bacillus subtilis and Pseudomonas aeruginosa both the organism have higher growth rate at pH 7 and Pseudomonas aeruginosa shows more growth than Bacillus. Pseudomonas aeruginosa shows higher growth rate as compare to Bacillus subtilis at 370C and the Pseudomonas aeruginosa shows better growth rate than Bacillus subtilis at 37°C.