Isolation and Characterization of Indole Acetic Acid (IAA), Gibberellic Acid (GA) Producing and Phosphate Solubilizing Microorganisms from Rhizospheric and Endosphaitic Nut Isolates of Groundnut Soil

Abstract

This research focuses on the isolation and characterization of microorganisms from the rhizospheric and endosphaitic regions of groundnut soil, with a specific emphasis on their ability to produce Indole Acetic Acid (IAA), Gibberellic Acid (GA), and phosphate solubilizing enzymes. The study aims to explore the potential of these microorganisms in enhancing plant growth and soil fertility.Groundnut fields were sampled, and microorganisms were isolated from both the rhizospheric and endosphaitic niches. Screening assays were conducted to identify strains capable of producing IAA and GA, while phosphate solubilization potential was assessed using qualitative and quantitative methods. Molecular characterization through 16S rRNA sequencing was performed to elucidate the taxonomic identities of selected strains.The results revealed a diverse array of microorganisms present in groundnut soil, with a significant proportion exhibiting the ability to produce IAA and GA. Additionally, a subset of the isolated strains demonstrated phosphate solubilization potential, indicating their role in enhancing nutrient availability to plants. Molecular characterization provided insights into the taxonomic composition of these beneficial microorganisms.The findings of this study have implications for agricultural practices, suggesting the potential utilization of IAA and GA-producing microorganisms in biofertilizer development to promote plant growth and crop productivity. Furthermore, the phosphate solubilizing abilities of certain microorganisms highlight their importance in soil fertility management and nutrient cycling.This research contributes to the understanding of plant-microbe interactions in groundnut soil and underscores the potential of these microorganisms in sustainable agriculture. By harnessing the beneficial properties of rhizospheric and endosphaitic microorganisms, innovative strategies can be developed to improve soil health, enhance crop yields, and promote environmental sustainability.