Inhibition of Salivary Amylase and Pancreatic Trypsin by Herbal and Microbial Extracts

Abstract

This study delves into the inhibition of salivary amylase and pancreatic trypsin, pivotal enzymes in carbohydrate and protein digestion, respectively, by herbal and microbial extracts. Salivary amylase initiates the breakdown of complex carbohydrates into simpler sugars in the oral cavity, while pancreatic trypsin plays a crucial role in protein digestion in the small intestine. Dysregulation of these enzymes can lead to metabolic disorders such as diabetes and obesity. Therefore, exploring natural inhibitors of these enzymes is of significant interest for potential therapeutic applications. In this research, various herbal and microbial extracts were investigated for their inhibitory effects on salivary amylase and pancreatic trypsin activities. Herbal extracts have long been recognized for their medicinal properties, and microbial extracts are gaining attention for their potential therapeutic benefits. The selection of extracts was based on their traditional use in folk medicine and emerging evidence supporting their bioactive properties. The inhibitory effects of the extracts on enzyme activities were assessed through rigorous assay procedures. Results indicated significant inhibition of both salivary amylase and pancreatic trypsin activities by select herbal and microbial extracts. The potency of inhibition varied among the extracts, suggesting diverse mechanisms of action. Moreover, some extracts exhibited dual inhibition, targeting both enzymes simultaneously, which could be advantageous in managing metabolic disorders comprehensively. The observed inhibitory effects of herbal and microbial extracts hold promise for the development of novel therapeutic interventions for metabolic disorders. By modulating the activity of key digestive enzymes, these natural extracts may help regulate postprandial glucose and lipid levels, thereby mitigating the risk of metabolic complications. Furthermore, the use of natural inhibitors may offer advantages over synthetic drugs, including fewer side effects and better tolerability. This study provides valuable insights into the potential of herbal and microbial extracts as inhibitors of salivary amylase and pancreatic trypsin. Further research is warranted to elucidate the underlying mechanisms of inhibition and to explore the clinical implications of these findings. Harnessing the bioactive properties of natural extracts could pave the way for innovative approaches in the prevention and management of metabolic disorders.