COMPARATIVE STUDY OF ACUTE AND SUB-CHRONIC TOXICITY IMPACTS OF CHLORPYRIFOS AND CYPERMETHRIN ON ADULT ZEBRAFISH (DANIO RERIO) ON BIOCHEMICAL, HISTOLOGICAL AND ACCUMULATION LOAD RESPONSES

Abstract

The toxicological effects of Chlorpyrifos (0,0-diethyl 0-3,5,6-trichloro-2-pyridyl phosphorothioate) and Cypermethrin (cyano-(3-phenoxy phenyl) methyl] 3-(2,2-dichloroethenyl)-2,2 dimethyl cyclopropane-1-carboxylate) on adult zebrafish (specimens exceeding six months of age) were examined in the current investigation. Selected adult male zebrafish were randomly and the acute and chronic toxic were exposed. The mortality rate of zebrafish under laboratory conditions up to 96h period. The toxicity tests showed the 96-h LC₅₀ values as 0.5, 0.10, 0.15, 0.20, 0.25, 0.30, 0.35, and 0.40 mg/l for Chlorpyrifos (CPS) and Cypermethrin (CP) respectively. Biochemical, Histopathological, and bioaccumulation loads were determined. The findings indicated that CPS and CP could swiftly accumulate within the physiological structure of fish during acute exposure, demonstrating a dose-dependent relationship. Furthermore, following a sub-chronic exposure period of 28 days, damage to the intestine and liver was noted. The organ impairment and metabolic disruptions induced by CPS and CP may contribute to the inhibition of zebrafish development. The objective of this investigation is to ascertain and compare the acute and sub-chronic toxicities of chlorpyrifos and cypermethrin pesticides