BIOCHEMICAL EFFECTS OF POLYHERBAL EXTRACTS ON SERUM PROTEIN AND KEY LIVER AND KIDNEY MARKERS IN MALE ALBINO WISTAR RATS

Abstract

Polyherbal formulations are increasingly being explored for their potential therapeutic benefits on various biochemical parameters. This study aims to evaluate the effects of a polyherbal extract on serum biochemical parameters in rats. Rats were divided into four groups, with Group I serving as the control and Groups II, III, and IV receiving 200 mg/kg.bw, 400 mg/kg.bw, and 600 mg/kg.bw of the polyherbal extract, respectively. Serum samples were collected and analyzed for protein levels, creatinine, SGOT, SGPT, and ALP. Statistical analysis was performed to determine the significance of the observed changes. The polyherbal extract significantly influenced serum protein levels. However, it did not significantly alter creatinine levels, with Group II (200 mg/kg.bw) showing a non-significant decrease to 0.45 ± 0.16 mg/dL. The extract had a notable impact on serum SGOT (serum glutamic-oxaloacetic transaminase) levels, with Group IV (600 mg/kg.bw) exhibiting the highest increase to 291.60 ± 10.70 U/L (p < 0.01), indicating a dose-dependent effect on liver enzyme elevation. In contrast, the extract had a mild and non-significant impact on serum SGPT (serum glutamic-pyruvic transaminase) levels, with Group IV showing a slight increase to 52.66 ± 2.60 U/L. Similarly, serum ALP (alkaline phosphatase) levels were not significantly affected, with only slight variations observed across the treated groups. These findings suggest that while the polyherbal extract can influence certain biochemical parameters, its effects on kidney function and some liver enzymes are not statistically significant at the tested doses.