EVALUATION OF A CONVENTIONAL PCR TECHNIQUE FOR THE MOLECULAR DETECTION OF ANISAKID PARASITES IN THREE DRAINING LIQUIDS OF CANNED MACKEREL

Abstract

Anisakids, which cause severe food allergies in children and the elderly, cannot be detected in hydrobiological commodities using sensory studies. Since parasites in canned fish are difficult to spot visually, this technique is not recommended. There needs to be an evaluation of molecular approaches to detecting Anisakid bugs in canned fish. Anisakid parasites were cultured and then fed to fish that had been experimentally infected. Total genomic DNA was extracted using the modified CTAB method from the control fluid that had been drained from the preserve following a thorough mixing of the contents. Two-fold amplification PCR was utilised to locate a 629bp region of the cytochrome c oxidase subunit II (COXII) gene. In total, 48 inoculation (with parasites) preserves, 48 control (uninoculated) preserves, and 48 preserves with additional parasites were examined. This demonstrated that the test's diagnostic sensitivity was 100% for preserves prepared using oil and commercial tomato sauce as the governing liquid, and 93.33%; for those prepared using water canning. Analysis and diagnosis were at the heart of the procedure.