Characterization and Identification of Atypical Yeast Species Causing Fungemia by MALDI-TOF MS Technique

Abstract

The incline healthcare services and improvements in technology have elevated resistance or confrontation amongst microorganisms. The microbial community has witnessed evolution by emerging variant yet similar strains which are hard to detect and treat. An upsurge in drug resistance has hit at last; overuse of drugs should be prohibited otherwise the future is about to come when there will be no antibiotic left to control an illness. Prescription of suitable drugs must be mandatory following accurate diagnostics to the disease causative agents or factors. The Matrix Assisted Laser Desorption Ionization-Time of Flight Mass Spectrometry (MALDI-TOF MS) represents new era of diagnostics and medical microbiology. This study focus on utilization of MALDI-TOF MS technique for characterization and identification of rare yeast species as clinical isolates screened from critically ill patients with fungal infection. The commencement of antifungal resistance in a wide spectrum of atypical yeasts causing fungemia in several hospitals of India is worrisome. The rising of hospital patients with compromised immunity are giving way to pathogenic fungi leading to death of thousands patients. At lower differential neutrophills count, the invasion of uncommon yeast Candida albicans (Fungi: Saccharomycetaceae) be able to root a systemic infection lead to surplus growth of pathogenic rare yeasts in many organs, and possibly fatal. At this stage, physicians’ often recommend anti-fungal drugs to keep the controlled yeast populace in patients. But over the time, main challenge to medical experts during treatment is the development or emergence of resistance in rare yeast species against the prescribed drugs, insisting medical and pharmaceutical scientists to design and formulate more potent drugs and device rapid, truthful diagnostic techniques