A Lectin Isolated From Marine Sponge Hyatella Cribriformis Exhibited Potential Antibacterial and Antiproliferative Activities

Abstract

Lectins are non-immune glycoproteins isolated from marine sponges shown to be many physiological, biological and pharma cological applications. Hyatella cribriformis, a marine sponge, yielded a lectin (HCL) that was partially purified using Sephadex G75 gel filtration chromatography.  When compared to the markers, the SDS-PAGE analysis of the fraction obtained from H. cribriformis produced two bands with molecular weights of roughly 35 kDa and 47 kDa, roughly. Using the well diffusion method, the antibacterial effects of HCL was investigated in several microorganisms. The positive control is gentamycin. Results demonstrated the antibacterial activity of HCL against S. aureus, P. aeruginosa, K. pneumoniae, and E. faecalis. The MTT test was used to evaluate the cytotoxicity of HCL against HeLa cell lines at varying doses (50–100 µg/mL). After 24 hours, HCL showed potential cytotoxicity against these cell lines in a dose-dependent manner, with an IC₅₀ value of 26.23 µg/mL. In clinical applications, HCL may therefore be a powerful therapeutic agent.