Standardization of Vaitaran Basti: Integrating Physicochemical Properties and HPTLC Fingerprinting for Enhanced Quality Control

Abstract

The standardization of Vaitaran Basti was conducted using three samples (200 ml each with Gomutra) through organoleptic, physicochemical, and HPTLC fingerprinting techniques to ensure quality and consistency. Organoleptic evaluation assessed sensory characteristics such as colour, odour, and texture. Physicochemical analysis revealed that the Loss on Drying (LOD) ranged from 73.84% to 74.22%, indicating consistent moisture content. The pH levels were slightly acidic, between 3.65 and 3.73. Specific Gravity (SG) values ranged from 1.037 to 1.045, and the Refractive Index (RI) was consistent at 1.314-1.315, reflecting uniform optical properties. The total solid content varied slightly, from 25.02% to 25.87%. HPTLC fingerprinting provided distinct chromatographic profiles for each sample, confirming the presence of essential chemical compounds. The results demonstrated a high degree of uniformity across all samples, ensuring reproducibility and reliability in the formulation of Vaitaran Basti. These findings support the therapeutic use of the standardized formulation in clinical settings.