In-vitro and In-vivo Evaluation of Antiarthritic, Anti-inflammatory and Analgesic Effect of Nanoemulsion loaded with Crinum latifolium leaves extract

Abstract

This study investigates the antiarthritic, anti-inflammatory, and analgesic properties of a nanoemulsion loaded with Crinum latifolium leaves extract through both in-vitro and in-vivo evaluations. Crinum latifolium, a plant known for its medicinal properties in traditional medicine, was selected for its potential therapeutic effects. The leaves extract was formulated into a nanoemulsion to enhance bioavailability and efficacy. In-vitro studies were conducted to assess the extract's ability of the nanoemulsion loaded plant extract to reduce the mediators and enzymes associated with arthritis. The antioxidant capacity was evaluated by DPPH method and showed the potent antioxidant activity of the emulsion. The nanoemulsion's stability and particle size distribution were also characterized. In-vivo evaluations were performed using established CFA induced arthritis, inflammation was evaluated by carrageenan induced paw edema, and pain was evaluated by eddy’s hotplate method. The results of different pharmacological evaluation were found to be significant at 1mg/kg of plant extract dose loaded in nanoemulsion. In the invitro evaluation at 1 mg/kg, the extract demonstrated 75.05% inhibition of hyaluronidase activity, The weight of the animas of test group was increased from 187.83±2.89 to 230.32±9.33 at the end of the study. The hematological parameters showed that the nanoemulsion have reduced the ESR significantly from 2.83±0.27 to 1.76±0.30** which indicated that nanoemulsion have reduced the infection in test animals in which the arthritis was caused. At the end of the study the nanoemulsion loaded with plant extract was found to be significantly effective, at the end of the study the inflammation of paws of animals were reduced and the arthritis was treated with significance (p< 0.025).   This study provides comprehensive evidence supporting the potential of Crinum latifolium leaves extract nanoemulsion as a promising candidate for managing arthritic conditions, offering combined antiarthritic, anti-inflammatory, and analgesic benefits. Further research is warranted to elucidate the precise mechanisms of action and to progress towards clinical trials.