A CBCT Assessment of Bone Density Changes During Accelerated Orthodontic Retraction of Canine by Platelet Rich Fibrin and Ascorbic Acid Augmented Platelet Rich Fibrin

Abstract

Objective: To compare and evaluate the effects of PRF and C PRF on the density of bone at a locally injected site.

Materials and Methods: A split-mouth randomized clinical trial was applied to 20 patients (>18 years of age) who required maxillary first premolar extraction. The right and left sides were randomized into quadrants receiving C PRF and PRF. Post extraction PRF and CPRF were repeatedly injected submucosally every 15 days. A CBCT of both the quadrants was taken pre-retraction(T0), post-retraction (T1), and 3 months(T3) post retraction and the density of bone at three sites; cervical third, middle third, and apical third were obtained in Grey Values was compared at the three-time intervals.

Results: T1 (post retraction) and T2 (3 months Post Retraction) in the Cervical region of the Canine between the two groups PRF and C PRF showed that the values were higher in the C PRF group with t values of 3.963 (p <0.001) and 3.692 (p<0.001) respectively. Analysis of bone density in the middle third of the canine root revealed a higher T2-T1 value in the PRF group compared to the C-PRF group. The comparison of canine root apical bone density between groups at T2-T1 showed a higher difference in the C-PRF group, but it was not statistically significant (t = 1.309, p = 0.198).

Conclusion: C PRF showed higher densities in the cervical region, while PRF led to a more significant increase over time in specific regions - the middle 1/3rd of the canine root and middle premolar region. Further research with larger samples and longer follow-ups is needed to confirm these trends and understand underlying mechanisms for effective bone regeneration and density promotion.

KEYWORDS

PRF, Canine retraction (CR), Orthodontic tooth movement (OTM), Platelet Rich Fibrin (PRF), Tooth Movement (TM), Growth factor (GF)

Objective: To compare and evaluate the effects of PRF and C PRF on the density of bone at a locally injected site.

Materials and Methods: A split-mouth randomized clinical trial was applied to 20 patients (>18 years of age) who required maxillary first premolar extraction. The right and left sides were randomized into quadrants receiving C PRF and PRF. Post extraction PRF and CPRF were repeatedly injected submucosally every 15 days. A CBCT of both the quadrants was taken pre-retraction(T0), post-retraction (T1), and 3 months(T3) post retraction and the density of bone at three sites; cervical third, middle third, and apical third were obtained in Grey Values was compared at the three-time intervals.

Results: T1 (post retraction) and T2 (3 months Post Retraction) in the Cervical region of the Canine between the two groups PRF and C PRF showed that the values were higher in the C PRF group with t values of 3.963 (p <0.001) and 3.692 (p<0.001) respectively. Analysis of bone density in the middle third of the canine root revealed a higher T2-T1 value in the PRF group compared to the C-PRF group. The comparison of canine root apical bone density between groups at T2-T1 showed a higher difference in the C-PRF group, but it was not statistically significant (t = 1.309, p = 0.198).

Conclusion: C PRF showed higher densities in the cervical region, while PRF led to a more significant increase over time in specific regions - the middle 1/3rd of the canine root and middle premolar region. Further research with larger samples and longer follow-ups is needed to confirm these trends and understand underlying mechanisms for effective bone regeneration and density promotion.