Antibacterial and Antioxidant Potential of Arisaema jacquemontii Blume from Manali, Himachal Pradesh

Abstract

Manali is not only a famous tourist place but also a treasure of manytraditional medicines. The antibacterial and antioxidant potential of leaves,tubers and fruit of Arisaema jacquemonti Blume were evaluated in differentextracts viz. acetone, chloroform, distilled water and methanol. The sampleswere collected from the area of Manali. Using Agar-well diffusion methodand different concentrations of extracts (25%, 50%, 75% and 100%), theantibacterial activity was tested against three Gram-negative bacteria(Salmonella typhi, Pseudomonas aeruginosa and Shigella dysenteriae) and twoGram-positive bacteria (Lysteria monocytogenes and Staphylococcus aureus).Streptomycin (5 μg/ml) was taken as a standard antibiotic. Percentinhibition of bacterial growth was calculated for each tested extract. Thetuber acetone, fruit methanol and leaf methanol extract were found to bemore effective against some the tested bacteria than the standard antibioticused. The chloroform and distilled water extract showed negligible results.IC50 value was calculated after 2, 2 Diphenyl-1-picrylhydrazyal (DPPH) freeradical scavenging assay to investigate the antioxidant potential at differentconcentrations (20, 40, 60, 80 and 100 μg/ml). Ascorbic acid at similarconcentrations was taken as standard (IC50 = 31.33μg/ml). The methanol-leaf extract was found to exhibit highest antioxidant potential with IC50value of 47.89μg/ml followed by methanol-tuber, water- fruit, acetone-fruitand methanol-fruit, acetone-tuber, acetone-leaf and water-leaf extracts. Theresults can be taken as a base for further analysis and isolation of activecompounds of this plant.