Effect of Zeatin and Indole-3-Acetic Acid in Vitro Culture of Butea Buteiformis Voig

Abstract

Butea buteiformis is a valuable tree with large trifoliate leaves with beautifulflower. The seeds were surface sterilized and cultured on half strengthMurashige & Skoog (1962) (MS) medium. Nodal explants obtained fromcultured were subcultured on different concentrations of zeatin (Zin) andIndole-3-acetic acid (IAA). The best proliferation of nodes and shoots wereobserved on the MS medium supplemented with 5 μM Zin and 1.0 M IAA.After 8 weeks of culture the propagated plants were acclimatized andtransferred to the sand box containing 1:1 soil and sand. Well rooted plantswere then established in the field. All the data collected were worked outstatistically with SPSS, a system of analytical procedure